A 1967 Ferrari 275 GTB/4 is valued at $3m-3.4m ahead of Gooding & Company's Scottsdale auction.
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The car is one of the most iconic models of the 1960s, with its flowing Pininfarina coachwork and powerful 300hp V12 engine.
Classics from Aston Martin, Shelby and Mercedes-Benz will also feature.
Auction house president David Gooding said: "Every one of these iconic sports cars delivers a remarkable and exciting driving experience.
"From the incredible designs to the distinct exhaust notes, there is an aura of something special when you get behind the wheel of each car."
The sale will take place on January 20-21.