Lady Gaga will consign her red Rolls-Royce Cormiche III to the May 17 Icons of Music sale at Julien's Auctions in Beverly Hills.
The lot is estimated to make around $30,000-50,000 for Musicares, a charity set up to provide healthcare and financial assistance for musicians.
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The singer bought the car in 2009 and most notably used it as a prop in her perfume launch in 2012.
A spokesman for Julien's told the MailOnline: "Lady Gaga bought the car because she liked it and has owned it for quite some time, I believe around five years.
"She doesn't collect cars so this may well be the only car she has, so it is a pretty rare item. The title is in her real name, Stefani Germanotta, and it's the first time a car belonging to her is going up for sale.
"I think it will bring in interest from Lady Gaga fans and Rolls Royce fans, but it might be something a car museum would want to display."
We have this mask worn by Lady Gaga that was designed by the creator of her infamous meat dress.
The major sale will also include a signed Beatles 65 album, George Harrison's 1962 Rickenbacker 425 and John Lennon's famous Hair Peace sign from his 1969 "Bed In" stunt.
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